2,6-Di-o-methyl Beta Cyclodextrin (DIMEB), has a well-defined structure and consists of a single component.

- It can be enhace the solubility of complexed substrates (substitute detergents and co-solvents)

- At low concentration it does not damage the microbial cells or the enzymes.

- It can intensify the enzymatic conversion of lipophilic substrates.

- It can improve the yield of product-inhibited fermentations.

- Organic toxic compounds are tolerated by microbes in higher concentrations.

- DIMEB complexes can substitute for mammalian serum in tissue cultures.

- Unstable and/or insoluble proteins can be dissolved and stabilized in aqueous solution.

Application 1: microbiological transformation

- Rapid formation of cholesterol complex

- Solubilization

- Enhanced conversion rate

- Decreased product inhibition

- Improved product stability

Application 2: biosynthesis by fermentation

- Enhanced production

- Diminished by-products formation

- Decreased product inhibition

Application 3: vaccine production

Complexation of fatty acids (growth inhibitors) results in enhanced cell growth and toxin production. DIMEB increase pertussis toxoid production 100-fold.

Application 4: serum-free culture media

A) .Cultivation of non-cultivable Mycobacterium leprae. B).Serum substitutes for lymphoblast cells. C). Non-cholesterol interacting fatty acid/CD complexs. D). Solubilization of lipids (fatty acids, cholesterol,phospholipids). E). No threat of prion proteins.

Application 5: artificial fertilization

-Improvement of the quality of semen by cholesterol supplumentation with cholesterol loaded methyl BCD.

-Enhancement of capacitation and fertility rate by preincubation of thawed spermas with methyl-BCD.